QUANTITATIVE COLONIAL GROWTH O1~ SINGLE S3 CELLS IN A MEDIUM CONTAINING SYNTHETIC SMALL MOLECULAR CONSTITUENTS AND TWO PURIFIED PROTEIN FRACTIONS* BY HAROLD W. FISHER,~ THEODORE T. PUCK, l~.D., AND GORDON
نویسندگان
چکیده
Previous studies on the molecular growth requirements for colony formation by single mammalian ceils in vitro (I-3) have demonstrated that these requirements are strongly influenced by the genetic constitution and degree of homogeneity of the cell strain employed; by the medium of previous cultivation of the cells and their treatment before inoculation; and by the treatment to which the macromolecuiar components of the medium had been subjected. When serum proteins were systematically fractionated, it was found that growth of single $3 HeLa ceils required two separate protein fractions, albumin and an ~globuiin (I). The present paper describes a medium containing synthetic micromolecular constituents and two purified protein fractions, which regularly produces colonies from single $3 HeLa cells plated in Petri dishes, with an effidency approaching I00 per cent.
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